Relationship Between the Detection of HPV E6/E7 mRNA and Cytological Features in Cervical Smears among Female Sex Workers in Chiangmai
DOI:
https://doi.org/10.14456/dcj.2013.31Keywords:
HPV E6/E7 mRNA test Pap smear, cervical cancer, female sex workersAbstract
The aim of this study was to evaluate the relationship between the result of HPV E6/E7 mRNA test and Pap smear test in target group sex workers. The subjects were 175 sex workers. Cervical cells were collected using liquid base media. HPV E6/E7 mRNA level in collected cervical cell was tested using Flow cytometer and the cervical cancer cell forming was determined microscopically by pap smear. Among 175 sex workers in the atudy, tTthere were 54 subjects (30.86%) with HPV E6/E7 mRNA test positive and 8 subjects (5.14%) with pap smear test positive. The relation between HPV E6/E7 and pap smear did not show in our study (Fisher exact p-value >0.05).It caused by the sensitivity difference between HPV E6/E7 test and Pap smear test. The sensitivity of HPV E6/E7 mRNA test and pap smear test should be further determined. In in order to evaluate the sensitivity of HPV E6/E7 mRNA test, the cervical cancer incidence among 54 subjects with HPV E6/E7 mRNA positive result should be followed using pap smear.
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2. Hamid NA, Brown C, Gaston K. The regulation of cell proliferation by the papillomavirus early proteins. Cell Mol Life Sci. 2009 May;66 (10):1700-17.
3. Jabbar S, Strati K, Shin MK, Pitot HC, Lambert PF. Human papillomavirus type 16 E6 and E7 oncoproteins act synergistically to cause head and neck cancer in mice. Virology. 2010 Aug 24.
4. Moody CA, Laimins LA. Human papillomavirus oncoproteins: pathways to transformation. Nat Rev Cancer. 2010 Aug;10(8):550-60. Epub 2010 Jul 1.
5. Agency for Health Care Policy and Research. Evaluation of cervical cytology: evidence report/ technology assessment (no.5) Rockville, MD: AHCPR, January 1999. Online monograph:
6. Method for specifically detecting tumor cells and their precursors in uterine cervical smears by simultaneously measuring at least 2 different molecular markers. Copyright 2004-2011 .
7. Detection, screening and diagnosis of HPV-associated cancers. http://www.freepatentsonline.com/privacy.html. 10 March 2011.
8. พวงทอง ไกรพิบูลย์. มะเร็งวิวัฒน์. วารสารสมาคมรังสีรักษา และมะเร็งวิทยาแห่งประเทศไทย. ปีที่ 3 ฉบับ กรกฎาคม- ธันวาคม 2550. หน้า 17
9. Xavier Castellsagu?. Natural history and epidemiology of HPV infection and cervical cancer. Gynecologic Oncology 110 (2008) S4-S7.
10. Schiffman M, Kjaer SK, Chapter 2: Natural history of anogenital human papillomavirus infection and neoplasia J Natl Cancer Inst Monograph 31 2003;14-9
11. zur Hausen, H. Papillomaviruses and cancer: from basic studies to clinical application. Nat Rev Cancer 2002, 2:342-50.
12. Irwin D. and Patterson B. Quantitative analysis of HPV E6/E7 mRNA expression in cells using flowcytometry (HPV ONCOTECT) improves positive predictive value for lesionson. Abstract from the clinical cytometry society 22nd annual meeting. Cytometry Part B (ClinicalCytometry) 72B:482-494 (2007)
13. Schwartz E, et al. "Structure and Transcription of the human papillomavirus sequences in cervical carcinoma cells. Nature 1985;314:111-114
14. Grundhoefer, D and B.K. Patterson 2001 Determination of liquid-based cervical cytology specimen adequacy using cellular light scater and flow cytometry. Cytometry 46: 340-344
15. Szarewski, et al, "Comparison of Predictors for High Grade Cervical Intraepithelial Neoplasia in Women with Abnormal Smears", CEBP 2008:17 (ii). Nov 2008
16. Ratnam, et al, "Clinical Correlation of APTIMA HPV Assay in Comparison with hc2 Test in Cervical Cancer Screening" ISSTDR Poster July 2009
17. Dockter, et al,"Clinical Performance of APTIMA HPV Assay for Detection of E6/E7 mRNA from HR HPVTypes in Liquid Based Cytology Specimens" ISSTDR Poster July 2009
18. S Dockter, J, et al. Clinical performance of the APTIMA? HPV Assay for the detection of high risk HPV and high grade cervical lesions J. Clin Virol 2009 Jul; 45 Suppl 1: 555-61
19. Kottaridi C, et al J. Inf Dis 2009 in press
20. Rodriguez AC., Et al. Prospective study of typespecific carcinogenic HPV infections over 7 years in Guanacaste, Costa Rica. Proceedings of the 24th International Papillomavirus Conference and Clinical Workshop. Beijing, China 2007. Abstracts book. Abstract 7B-02.
21. M?ckel J, Clad A, Endres A-S and Schneider V. HPV E6/E7 mRNA transcripts as predictors of high-grade epithelial cervix dysplasia. Diagnostic Pathology 2007.
22. Fiedler M. et al. High level HPV-16 E7 oncoprotein expression correlates with reduced pRb-levels in cervical biopsies. The FASEB Journal. Vol. 18 July 2004: 1120-22.
23. ปราณี ลี้ธนะชัย. การพัฒนาวิธีการตรวจหา mRNA ด้วยวิธีการเพิ่มปริมาณกรดนิวคลีอิกเพื่อใช้พยากรณ์ความเสี่ยงของการเกิดมะเร็งปากมดลูกในผู้ติดเชื้อ Human papillomavirus. Copyright @ 1999 National Center for Genetic Engineering and Biotechnology [BIOTEC]
24. จิรานุวัฒน์ บาริศรี และคณะ. การศึกษาการแสดงออกของ E6/E7 mRNA ของเชื้อไวรัส HPV ที่เป็นสาเหตุของการเกิด มะเร็งปากทวารหนักในกลุ่มชายที่มีเพศสัมพันธ์กับชายที่ติดเชื้อและไม่ติดเชื้อเอชไอวี. การสัมมนาระดับชาติเรื่องโรคเอดส์ ครั้งที่ 13, 29-31 มีนาคม 2554.
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