Detection of Y-STR Touch DNA for Personal Identification on a Wooden Knife Handle Used as a Stabbing Weapon

Authors

  • Boonpim M Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University
  • Kanchai C Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University
  • Bhoopat T Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University

Keywords:

Touch DNA, Y-STR, transfer, knife

Abstract

Objectives The purpose of this study was to examine the effectiveness of Y-STR profiling of touch DNA from a woodenhandle knife used as a stabbing weapon.

Methods A group of 60 male volunteers were asked to stab a foam box with a wooden-handle knife held firmly in their hand. DNA from the surface of the knife handles was extracted using a Qiagen® QIAamp DNA Investigator kit and amplified using a modified Investigator® Argus Y-12 QS protocol.

Results The touch DNA profiling found that 6 of 60 of full profile samples (10%) gave a full profile and 21 samples (35%) showed useful partial profiles. No significant difference was observed between right- and left-hand dominant volunteers (p = 0.5615 95% CI = -24.6237 to 44.1160, Chi-squared test of proportion).

Conclusion Y-STR profiling of touch DNA is suitable for use in conjunction with other personal identification methods.

Downloads

Download data is not yet available.

Downloads

Published

2020-07-01

How to Cite

1.
M B, C K, T B. Detection of Y-STR Touch DNA for Personal Identification on a Wooden Knife Handle Used as a Stabbing Weapon. Chiang Mai Med J. [Internet]. 2020 Jul. 1 [cited 2022 Jun. 29];59(3):121-5. Available from: https://he01.tci-thaijo.org/index.php/CMMJ-MedCMJ/article/view/245257

Issue

Section

Original Article